Quixotic rewriting in William Goldman's The princess bride (1973)

Trabajo de fin de Grado. Grado en Estudios Ingleses. Curso académico 2021-2022En La Princesa Prometida (1973), William Goldman explora la dualidad entre vida y literatura, parodiando las convenciones propias de los cuentos de hadas, pero a su vez reafirmando el valor de las nociones de “gran aventura” y “amor verdadero,” inalcanzables en la realidad. Al articular una crítica tanto de la literatura como de la realidad, la novela puede ser vista como una reconstrucción de las ideas presentadas en el Don Quijote de Cervantes. Este ensayo pretende analizar la aplicación del patrón quijotesco en esta obra, en la que el acto de reescritura puede ser entendido como un intento de recobrar lo romántico en el espacio literario. En primer lugar, presentaré las nociones de reproducción tanto quijotesca como cervantina. En segundo lugar, profundizaré en la forma en que el quijotismo del héroe de Cervantes y su misión son transferidos al narrador y su proyecto editorial en la novela de Goldman. Finalmente, se examinarán las implicaciones ambivalentes de esta actuación quijotesca, en virtud de la cual se lleva a cabo una parodia del género literario, pero también una crítica de la degradada condición del mundo, lo que identifica a Goldman como heredero de la tradición Cervantina.In The Princess Bride (1973), William Goldman explores the conflicting duality of life and literature, mocking fairy-tales conventions, but at the same time reaffirming the importance of “high adventure” and “true love,” values that are unattainable in real life. Articulating a critique on both literature and reality, this novel can be regarded as a reconstruction of the ideas presented in Cervantes’ Don Quixote. This paper aims to analyse the application of the quixotic formula to the novel, in which the act of re-writing can be understood as a quixotic attempt to recover the romance within literary space. In the first place, I will introduce the notions of quixotic and Cervantine reproduction. In the second place, I will analyse the ways in which the quixotism of Cervantes’ hero and his quest are transferred to the narrator of the work and to his editorial project in Godman’s novel. Finally, the ambivalent dialogical implications of this quixotic stance will be examined, namely the parody of a genre, but also the satire of the debased nature of the world, which identifies Goldman as an heir of the Cervantine tradition

Aplicación de la realidad aumentada en videojuegos

La computación grafica es un campo de las ciencias de la informática, la cual busca investigar sobre técnicas y metodologías proporcionadas por la matemática, geometría, física, álgebra lineal, óptica, etc. Teniendo como objeto el generar imágenes visuales artificiales y completar o cambiar la información visual y espacial reconocida del mundo real. Esta es una de la ares de la ciencias de la informática más interesantes que hay, su contribución a sido fundamental para el desarrollo tanto de hardware como de software, ya que sus implementaciones requieren recursos cada vez mayores. El uso de gráficas por computador ha sido de gran ayuda para desarrollos, con el fin de contribuir en diversos campos de la ingeniería e investigación científica. La Computación Gráfica cubre áreas muy diversas, que abarcan desde la visualización científica o de ingeniería, Gráficos estadísticos, Cartografía, medicina, diseño Asistido por Computadora (CAD) et

Solución de Dos Estudios de Caso Bajo el Uso de Tecnología CISCO

Este trabajo tiene el desarrollo de dos escenarios propuestos en el diplomado de profundización de cisco a partir del simulador cisco packet tracer.This work has the development of two scenarios proposed in the diploma of deepening of cisco from the simulator cisco packet tracer

Analysis of the waste selective collection at drop-off systems: Case study including the income level and the seasonal variation

There are several factors which have an influence in the selective collection of the municipal waste. To define a selective collection system, the waste generation pattern should be firstly determined and these factors should be analyzed in depth. This paper tries to analyze the economic income level and the seasonal variation on the collection and the purity of light-packaging waste to determine actions to improve the waste management plan of a town. In the first stage of the work, waste samples of the light-packaging containers were collected in two zones of the town with different economic characteristics in different seasons during one year. In the second stage, the samples were characterized to analyze the composition and purity of the waste. They were firstly separated into four fractions: metals; plastic; beverage cartons; and misplaced materials. The misplaced fraction was in its turn separated into cardboard, rubber and leather, inert waste, organic matter, paper, hazardous waste, clothes and shoes, glass and others. The plastic fraction was separated into five types of plastics and the metal fraction into three. In the third stage, the data have been analyzed and conclusions have been extracted. The main result is that the quality of the light-packaging fraction collected in these zones during both seasons were similar. This methodology can be extrapolated to towns with similar characteristics. It will be useful when implementing a system to collect the waste selectively and to develop actions to achieve a good participation in the selective collection of the waste

The implementation of neuro-linguistic programming (nlp) techniques in primary graders’ oral performance in english from the instituto kennedy in Pereira

Este proyecto tiene como objetivo mostrar los efectos de la implementación de cinco técnicas de Programación Neurolingüística: anchoring, mirroring, visualization, mind mapping and positive verbal reinforcement para impactar en la habilidad del habla en inglés de 37 estudiantes de grado quinto de un colegio de la ciudad de Pereira. Los practicantes expusieron a los estudiantes a estas técnicas durante 11 clases con el fin de recolectar y analizar información relacionada con crecimiento profesional de los profesores, respuestas y producción lingüística de los estudiantes a través de instrumentos como producciones de los estudiantes, observaciones y diarios de campo. Por lo tanto los profesores diseñaron y reflexionaron sobre las lecciones. Consecuentemente, los resultados obtenidos de la implementación de este proyecto indicaron que la mayoría de las técnicas aplicadas afectaron positivamente el desempeño de los estudiantes en actividades del habla ya que produjeron el vocabulario y las estructuras esperadas en estas actividades. Además, se comprobó que estas técnicas despertaron la motivación en los estudiantes en la habilidad del habla debido a la activa participación que ellos estuvieron durante las actividades. Todo esto en conjunto, tuvo un impacto positivo en el aprendizaje de inglés

Asociación de hiperplasia tonsilar y bruxismo del sueño en niños y niñas de 4 a 12 años de la clínica odontológica de la universidad Andrés Bello

Tesis (Cirujano Dentista)El Bruxismo del Sueño es un trastorno relacionado a la obstrucción de la vía aérea superior, por lo que la Hiperplasia Tonsilar se podría asociar como una de sus causales. Objetivo: Determinar la asociación entre hiperplasia tonsilar y bruxismo del sueño en niños y niñas de 4 a 12 años. Método: Estudio epidemiológico cuantitativo descriptivo transversal. La muestra está conformada por 162 participantes de entre 4-12 años atendidos de la Clínica Odontológica de la Universidad Andrés Bello, sede Santiago, mediante muestreo no probabilístico consecutivo. Se aplicaron tres cuestionarios asociados a Bruxismo del sueño posible, ronquidos, trastornos respiratorios del sueño (Pediatric Sleep Questionnaire) y sospecha de rinitis alérgica (1). La modalidad fue presencial autoreportada por los cuidadores. Se realizó un examen clínico previa calibración, se evaluarón las escalas de Brodsky y Mallampati. Resultados: Del total de la muestra el 54.2% presentaba bruxismo del sueño e Hiperplasia Tonsilar de forma simultánea. Estas variables no presentan asociación estadística (x2 p:valor: 0.131). Sin embargo, en los hombres si hay asociación estadística (x2 p:valor: 0,007). Conclusiones: El Bruxismo del Sueño es un trastorno multifactorial y no es asociable a la Hiperplasia Tonsilar por si sola

El aporte de los lineamientos del marco de trabajo de la AOTA, en los programas de transición a la vida adulta

Tesis (Terapeuta Ocupacional)El proyecto de investigación abordó el aporte de la Terapia Ocupacional basándose en los lineamientos del Marco de Trabajo de la AOTA en la estrategia de Transición a la Vida Adulta, a través de un análisis exploratorio-descriptivo de dos escuelas especiales que implementen el programa, en donde La Escuela Especial Andalué de Maipú incorpora el trabajo de un Terapeuta Ocupacional y por otro lado en La Escuela Especial La Espiga de Puente Alto no cuenta con la presencia de un Terapeuta Ocupacional, a fin de poder identificar las estrategias de trabajo del programa Transición a la Vida Adulta que cuentan las dos escuelas especiales, así mismo se abordaron los lineamientos de la educación diferencial y del Marco de Trabajo de la AOTA en la implementación de la estrategia antes mencionada, como también se identificó si sus lineamientos comparten estrategias, lo que nos permitió evidenciar el trabajo que realiza el Terapeuta Ocupacional en los contextos educacionales, demostrando el aporte en las áreas que abarca el programa, con los estudiantes de las escuelas especiales

Uncovering and Functional Analysis of Novel Genes and Potential Genetic Modifiers for Neuromuscular Disorders

The inherited Neuromuscular Disorders (NMDs) is an umbrella term that encompasses a plethora of diseases that affect the functioning of the muscles and/or their underlying nervous system control. Although individually uncommon, NMDs collectively are no longer considered rare diseases. Only in Europe, approximately 300,000 people are yearly diagnosed with one NMD. The diagnosis of a neuromuscular condition –successful in less than 50% of the cases- is often devastating to patients and their relatives. No cure is available for most of these disorders, and the few available treatments will at best delay disease progression. To find treatments and to develop methods for the early diagnosis of NMDs is a goal of highest importance, and the recent advances in the NGS technologies are the platform to reach this objective. Particularly, the implementation of WES has not only minimized the time and costs of genetic diagnostics but also provided unique opportunities for the exploration of gene function in NMDs pathogenesis. The uppermost goal of this PhD project was to identify and characterize novel NMDs-causative genes. Thereby, two novel NMD-causative genes were investigated in this thesis. First, CHP1 (Calcineurin Homologous Protein-1) was identified as a novel causing gene of Autosomal Recessive Cerebellar Ataxia (ARCA) and second, VAChT (Vesicular Acetylcholine Transporter, encoded by SLC18A3) was analysed in the context of distal Hereditary Motor Neuropathy (dHMN). Following a combination of WES and linkage analysis, we identified a biallelic 3-bp deletion (p.K19del) in CHP1 that co-segregates with a complex ARCA in two siblings of a consanguineous family exhibiting motor neuropathy, cerebellar atrophy and spastic paraparesis. CHP1 was selected as a top disease candidate since: (I) the mutation affects an amino acid highly conserved across species, (II) a point mutation in murine Chp1, causing aberrant splicing and reduced full-length Chp1 transcripts, leads to Purkinje cells loss and ataxia, (III) CHP1 assists posttranscriptional glycosylation and membrane localization of NHE1, a major neuronal Na+/H+ exchanger, (IV) KO of mouse Nhe1 cause ataxia and loss-of-function mutation in NHE1 (encoded by SLC19A1) cause ataxia-deafness Lichtenstein-Knorr syndrome (LINKS). Therefore, we hypothesized that a mutation in CHP1, as a crucial regulator of NHE1, could impair expression and targeting of the exchanger resembling the pathogenesis in mice and humans. To further uncover other families carrying CHP1 mutations, we performed a focused screening for CHP1 variants in two large ARCA and NMD cohorts (approximately 1000 exomes). No additional variants fulfilling or selection criteria were found, which emphasizes on the scarcity of CHP1 variants and the reduced tolerability of CHP1 for mutations. With the purpose to assess the functional consequences of the CHP1-K19del mutation on protein function, size exclusion chromatography (SEC), protein fractionation, 3D-protein modelling, fluorescence microscopy and in vivo zebrafish modelling were performed. We demonstrated that mutant CHP1 fails to integrate into functional protein complexes and is prone to aggregate, thereby leading to diminished levels of soluble CHP1 and reduced membrane targeting of NHE1 both in neuronal and non-neuronal cells. To analyze the pathogenic consequences of the hypomorphic CHP1-K19del mutation in vivo, we used morpholinos (MOs) to inhibit chp1 translation in zebrafish. Closely resembling the clinical features of the ARCA-affected siblings, chp1 downregulation in zebrafish led to cerebellar hypoplasia, Caudal Primary Motor Neuron (CaP-MN) defects and spastic trunk movements. All defects were ameliorated by co-injection with WT, but not mutant, human CHP1 mRNA, hence demonstrating both the specificity of the chp1-MO-induced phenotypes and validating the effect of CHP1-K19del on protein expression and/or function in vivo. Altogether, our results identified CHP1 as a novel ataxia-causative gene in humans, further expanding the spectrum of ARCA-causative loci, and highlight the crucial role of NHE1 within the pathogenesis of these disorders. Moreover, we conducted functional analyses to ascertain the functional basis of a dHMN presented by a family with cranial nerves palsy and vocal cord paresis as an initial feature of a non-progressive infantile onset dominant dHMN. WES analysis of this family led to the identification of a de novo dominant missense mutation (c.439 G>A, p.D147N) in VAChT. The mutation occurred first in the affected mother and was inherited by her affected daughter. VAChT controls the storage of the neurotransmitter Acetylcholine (ACh) by synaptic vesicles, hence it plays a fundamental role in cholinergic neurotransmission and therefore, in the plethora of processes reliant on it, which include: neuronal development and maturation, synaptic transmission and plasticity, patterning of the neuromuscular junction (NMJ), among others. The potential effect of the D147N mutation on VAChT subcellular distribution was analysed in neuron-like NSC-34 cells transiently overexpressing WT or mutant VAChT-GFP tagged proteins. No significant differences were observed in protein expression or localization, thus a detrimental effect of VAChT-D147N mutation at this level was not possible. This prompted us to further examine potential defects either in MN development and axonal outgrowth. Capitalizing once again on the advantages of the zebrafish for the modelling of human neurodegenerative disorders and further considering the evolutionary conservation of both VAChT and the D147 residue across species, the effect of WT and VAChT-D147N OE on CaP-MN outgrowth was analysed in detail. Although our findings were not conclusive at discerning the pathogenicity of the VAChT–D147N in vivo, we observed an axonal migration phenotype that could potentially underlie impairments at the NMJ level. In the light of the novel association of VAChT mutations as causative of myasthenia syndromes, follow-up studies will be performed in order to conclusively confirm the pathogenicity of the VAChT-D147N in a CaP-MN-independent context. The biological function of CHP1 was of further relevance within the scope of this doctoral project, since CHP1 is currently subject of study as potential modifier of Spinal Muscular Atrophy (SMA). Pre-existing evidence from our research group indicates that Chp1 downregulation –within a certain threshold- restores neurite outgrowth and impaired endocytosis (a key pathway disturbed in SMA) in Smn1-depleted NSC-34 cells. Thereupon, this thesis further aimed to validate reduction of Chp1 as potential SMA protective modifier in a zebrafish model of SMA, as a first in vivo approach. Here, we demonstrate that Chp1 downregulation ameliorates the CaP-MN axonal outgrowth defects of Smn-deficient fish larvae. These findings are in concordance with prior validation studies of two other human SMA modifiers –PLS3 and NCALD- in zebrafish, which despite their different function and mode of action (upregulation or downregulation, respectively) exert similar effects on CaP-MN morphology whereby restoring the CaP-MN phenotype of smn morphants in a highly comparable range. Altogether, our findings together with the preliminary findings aforementioned, strongly support CHP1 reduction as a promising therapeutic target for a combinatorial treatment, i.e. together with SMN restoration, counteracting SMA pathology

Implementation of a custom time-domain firmware trigger for RADAR-based cosmic ray detection

Interest in Radio-based detection schemes for ultra-high energy cosmic rays (UHECR) has surged in recent years, owing to the potentially very low cost/detection ratio. The method of radio-frequency (RF) scatter has been proposed as potentially the most economical detection technology. Though the first dedicated experiment to employ this method, the Telescope Array RADAR experiment (TARA), reported no signal, efforts to develop more robust and sensitive trigger techniques continue. This paper details the development of a time-domain firmware trigger that exploits characteristics of the expected scattered signal from an UHECR extensive-air shower (EAS). The improved sensitivity of this trigger is discussed, as well as implementation in two separate field deployments from 2016-2017

Narco-mantas o el confín de lo criminal

Este artículo recurre a la teoría de actos discursivos de J.L. Austin para realizar un análisis textual de las “narco-mantas”, o mensajes públicos firmados por organizaciones criminales. En estos textos breves y fragmentarios se percibe la formación de un discurso político incipiente. Hay en ellos una idea de justicia, la intención de legitimarse frente a un público anónimo, así como intentos frágiles de hablar desde un “nosotros” popular. En la interpretación de la violencia que las narco-mantas buscan imponer está en juego la distinción entre lo criminal y político